Glioblastoma (GBM), the most aggressive central nervous system tumor, invades brain tissue while experiencing diverse mechanical stimuli. This study explores how cytoskeletal dynamics influence GBM mechanical properties under deformation of a magneto-active substrate [1]. Using mechanical measurements and immunofluorescence, we show that actin dominates mechanics in relaxed states, while vimentin governs responses under strain. Vimentin knock-out (KO) cells exhibit softening and increased nuclear deformation post-deformation. The experimental results motivate a constitutive model that describes the dynamics of the cytoskeleton components interplay, and their impact on cell mechanics.